Descriptions

Specifications

Product Features

촬영시 3가지 모드로 손쉽게 촬영이 가능하고, 다양한 파라메터를 직접 조정해서 더욱 깔끔하고 명확한 이미지 획득이 가능합니다.
UV laser protective plate가 있어서 gel cutting을 할때 편리하게 그리고 안전하게 할 수 있습니다.
 

Technical Specifications and Precautions 

Functional Description

Operating Procedures

Power On

Plug in the power cord and turn on the power switch at the back of the instrument. The industrial computer will start up.

Sample Loading

Protein Gel: Open the instrument door, take out the white sample tray, place the prepared text sample on the tray, and then place the tray flat in the groove inside the instrument dark box. Close the instrument door.

Nucleic acid gel: Open the instrument door, place the sample on the UV-transmitting glass of the UV light source module. There are markings on the UV glass surface indicating the sample placement area.

Launching the Imaging Software

Preview and Capture Pages

The preview and capture page includes status display information,  camera settings, lens settings,  mode settings, image saving and cropping, etc.

The top bar includes UV light source status indicator, keyboard open button, minimize, window restore, exit gel imaging; file path input for saving the location of the image, file name input for saving the name of the image.

The middle window displays real-time images taken by the camera.

The bottom bar includes camera/control board/mobile disk connection status display information,  image format selection for saving (TIFF/JPEG/BMP), save image button, open image path, image crop button.

The right sidebar is for camera settings, mode settings, and lens settings area, details are as follows:

Video Settings Double-clicking “Video Setting” will center the image

Contrast    Brightness  adjustment,  range  -100~ 100,  default value  0,  increasing  the value  makes  the  image brighter, decreasing the value makes the image darker

Exposure Set exposure time in milliseconds, default time 350ms, increasing the time makes the image brighter Gain Set gain value, default value 500%, range 100%~5000%, increasing the gain makes the image brighter

Auto Exposure Automatic exposure mode, the camera automatically adjusts exposure time and gain Manual Exposure Manual exposure mode, manually set exposure time and gain

Inverse Color Black and white color inversion

Lighting Turn on/off the LED lighting, used for shooting protein gel mode

UV Light  Turn on/off the UV light source, can only operate on/off when the door is closed, used for shooting nucleic acid gel mode

Gel Cutting Turn on/off the gel cutting mode, can only operate on/off when the door is open, when opening gel cutting, the UV light source will also automatically turn on, used for cutting gel, the UV protection shield must be placed before opening gel cutting

Adjustment Mode Select the zoom, focus, and aperture adjustment range of the electric lens

Coarse Adjustment   Larger adjustment steps, zoom adjustment step 200, focus adjustment step 1000, aperture adjustment step 200, when the image is completely blurry, coarse adjustment can quickly make the image relatively clear

Fine Adjustment  Moderate adjustment steps, zoom adjustment step 50, focus adjustment step 250, aperture adjustment step 50, after coarse adjustment, fine adjustment can make the image clearer

Super Fine Adjustment  Smaller adjustment steps, zoom adjustment step 5, focus adjustment step 25, aperture adjustment step 5, can make the image in the clearest state

Zoom  Increase and decrease field of view by clicking “-” and “+” buttons Focus Adjust image clarity by “-” and “+” buttons

Aperture Adjust image brightness by “-” and “+” buttons

Capturing Process

1.Select automatic exposure, default contrast is 0, default exposure is 350, and default gain is 500 .

2. For nucleic acid gel: Click on the UV light, turn on the UV light source, then the bands can be seen in the window.

For protein gel: Click on the illumination, turn on the bright field light source, then the bands can be seen in the window.

3.Adjust the “zoom” to fill the field of view with bands, or adjust to the experimenter ‘s desired field of view.

4.Adjust the “aperture,” first adjust to the maximum, then click ” — ” twice for fine adjustment, and the aperture is set to 3 .

5.Adjust the “focus ” to make the image as clear as possible; in rare cases, if the results of fine adjustment and super-fine adjustment are still  not clear enough, adjust to  reduce the  “aperture,” then focus to obtain the clearest image.

6.Enter the file path and file name, click “Save Image” to save the current image.

6. If only part of the bands is needed, click on ” image crop” to enter the image cropping page. Slide the screen from the upper left corner of the desired band to its lower right corner, and a red box will appear to outline the band. Double-click on any position inside the red box to zoom in the band to fill the entire window; Horizontal flip and vertical flip can be used to flip the band along the horizontal and vertical directions, while restore can return the band to its original state before flipping; After adjusting the band to the desired state, enter the file path and file name, then click “Save Image ” to save the current image; Click ” Exit ” to return to the preview and shooting page. If any adjustments are made to the image on the results page

Gel Cutting

1.Open the instrument door, the UV light source will automatically turn off.

2. Pull out the drawer of the UV light source, place the UV protective shield diagonally between the drawer and the instrument to shield the eyes from the UV light source.

3.Click on “Gel Cutting,” the UV light source will turn on. At this point, the bands of the nucleic acid gel can be observed, and the gel cutting action can be performed.

Product Packing List

Descriptions

SCG-W3000 Chemiluminescence Imaging System

1. Whether the signal is weak or strong, a single imaging session can deliver your WB results.

• For weak signals, you can directly extend the imaging time based on the original image for further imaging.

• For strong signals, you can adjust the timeline to retrieve any result image from the imaging duration.

2. Supports simultaneous imaging of up to 10 membranes (or even more), improving instrument efficiency—1 device equals 10!

3. Dual-camera system:

• A color camera captures markers with true-to-life colors for greater authenticity.

• A monochrome camera captures bands with high sensitivity, delivering superior performance for weak signals.

4. Marker optimization function:

• Eliminates nonspecific band signals caused by prestained markers and antibody interactions.

• Adjusts overexposed or underexposed chemiluminescent marker signals.

5. Source file storage allows flexible adjustments anytime:

• If bands appear too thick or too thin after the experiment, you can easily fine-tune the results on your computer using the source file function!

Work with the ECL Chemiluminescence Kit (G2161-200ML), it meets the exposure needs of high, medium, and low-concentration samples. Enjoy a better WB imaging process and achieve superior WB imaging results!

SWE Imaging Grayscale Analysis Software-SCG-W2000 SCG-W3000-3.3.2-win32-x64-setup.exe

Download link from Google Drive:https://drive.google.com/file/d/1IoXazML9PDHoN4HqOYm-t3C2bVFvQ_rt/view?usp=drive_link

SOFTWARE USER MANUAL:

SWE Imaging Grayscale Analysis Software-SCG-W2000 SCG-W3000.pdf

Notes:

1. Do not touch or scratch the internal lens of the dark box with hands or sharp objects.

2. After placing the sample, ensure the instrument door is properly closed to prevent external light from entering and affecting results.

3. During imaging, avoid shaking the workbench or instrument to prevent compromised image quality.

4. Observe electrical safety—do not pull the power cord or move the instrument during operation.

5. After experiments, clean the dark box thoroughly, removing all samples and residues.

Parameter Comparison

Specifications

Operation Steps
Plug the power cord of the grinder into a grounded 220V power outlet, and turn on the power switch at the back of the grinder.
After the startup interface is displayed, wait for about 5 seconds to enter the main operating interface.
Once the grinder is turned on, the cooling system starts running. Place the grinding tubes containing the samples, tighten the adapter, and cover the top of the grinder.
Set the operating frequency, time, and number of cycles. Click the “Temperature Selection” button to choose the temperature mode and set the desired temperature for the grinding chamber or the cooling platform.
Click the start button to begin the grinding process. The instrument will automatically stop once the timer reaches zero.

Equipment Maintenance
Perform the following routine maintenance after each use to ensure the reliable operation of the grinder.
Make sure to turn off the power and unplug the power cord before cleaning.
If solvents, salt water, acidic or alkaline solutions are spilled on the grinder, immediately wipe them clean with a damp cloth to avoid damaging the instrument.
Do not use high-pressure sterilization on any part of the grinder, including the adapter.
The components of the adapter should be cleaned after use. Use an appropriate cleaning agent, rinse with distilled water, and then wipe dry with a paper towel.
After using an appropriate cleaning agent, wipe the grinder clean with a soft cloth.
The following disinfectants and cleaning agents are recommended for cleaning the grinder and adapter:
a) Ordinary cleaning agent
b) Neutral detergent
c) 70% alcohol content 

 Equipment List

DNA Extraction

(This condition is for reference only, and can be adjusted at any time during the experiment according to the grinding effect.)

 RNA Extraction

(This condition is for reference only, and can be adjusted at any time during the experiment according to the grinding effect.)

For RNA extraction, the grinding time is extended compared with that of protein extraction, and RNA extraction solution is added to the grinding, the specific grinding time depends on the amount and type of tissue to be ground. Samples difficult to break can be repeated grinding 2-3 times, the specific situation can be adjusted appropriately according to the experiment.
Strong and Tough Tissue Grinding
For samples that are difficult to grind (e.g. cartilage, hair, etc.), we recommend using a 2mL grinding tube. Cut the sample into 1-2mm pieces, add the appropriate number of grinding beads, cover the tube tightly and freeze in liquid nitrogen for 2-3min without extracting solution, dry grind for 60 seconds at 70Hz, then add extracting solution to the tube and repeat the grinding once. If the grinding result is not satisfactory, the tube can be frozen again and the above experimental steps can be repeated.
Bone Tissue Grinding
For bone tissue, use the special steel grinding jar and adapter. The bone tissue is first frozen in liquid nitrogen for 5-10min, then put into the grinding jar, add the steel beads, and grind for 180 seconds at 70Hz (set cycle grinding). After grinding, take out the finished tissue, put it into a 2mL grinding tube, add the extraction solution, two 3mm grinding beads and homogenize for 60 seconds.