Descriptions

Prestained Protein Marker II (10-200 kDa)

G2058-250UL X2

Prestained Protein Marker II consists of ten high-purity and pre-stained recombinant proteins, which is convenient for monitoring of electrophoresis and transfer efficiency. It is suitable as a protein molecular weight standard for SDS-PAGE and Western Blot.

The bands display in Tris-Glycine gel range from 10-200 kDa (~10, ~18, ~23, ~30, ~42, ~55, ~75, ~110, ~140, ~200 kDa), with orange-red band at 75 kDa, rose-red band at 10 kDa, and blue bands at the others.

Prestained Protein Marker Ⅳ (8-200 kDa)

G2083-250UL x2

This product, Prestained Protein Marker IV, is composed of 11 highly pure and pre-stained recombinant proteins. It indicates a molecular weight range of 8-200 kDa in Tris-Glycine gel (~8, ~13, ~20, ~30, ~40, ~55, ~68, ~90, ~110, ~140, ~200 kDa), with the 90, 30, and 8 kDa markers appearing as pink bands, while the others appear as blue bands. This allows for the dynamic observation of protein electrophoresis or the assessment of protein transfer efficiency. It is suitable as a protein molecular weight standard for SDS-PAGE and Western blot.

Prestained Protein Marker VI (55-320 kDa) – 고분자용

G2085-250UL

Prestained Protein Marker VI consists of 8 high-purity and prestained recombinant proteins, which is convenient for monitoring of electrophoresis and transfer efficiency. It is suitable as a protein molecular weight standard for SDS-PAGE and Western Blot.

The bands display in Tris-Glycine gel range from 55-320 kDa (~55, ~65, ~90, ~130, ~165, ~210, ~270,  ~320 kDa), With orange-red band at 90 kDa, and blue bands at the others.

Prestained Protein Marker Ⅶ (8-195 kDa) 

G2087-250UL x2

Prestained Protein Marker Ⅶ is a prestained mixture of ten high-purity recombinant proteins (no His-tag), The bands display in Tris-Glycine gel range from 8-195 kDa (~8, ~13, ~20, ~28, ~40, ~55, ~70, ~105, ~140, ~195 kDa), of which 70 kDa and 8 kDa are rose-red bands, others are blue bands. which is convenient for monitoring of electrophoresis and transfer efficiency. It is suitable as a protein molecular weight standard for SDS-PAGE and Western Blot.

Prestained Protein Marker Ⅷ (8-270 kDa) 

G2089-250UL

Prestained Protein MarkerⅧ is a prestained mixture ofeleven high-purity recombinant proteins, The bands display in Tris-Glycine gel range from8-270 kDa (~8,~13,~20, ~30, ~40, ~55, ~68, ~90, ~130, ~165, ~270 kDa), of which90 kDa, 30 kDa and 8kDa are rose-red bands,others are blue bands. which is convenient for monitoring of electrophoresis and transfer efficiency. It is suitable as a protein molecular weight standard for SDS-PAGE and Western Blot.

Prestained Protein Marker Ⅸ (2-40 kDa) – 저분자용

G2090-250UL

Prestained Protein Marker Ⅸ is a prestained mixture of seven high-purity recombinant proteins and peptides, The bands display in Tris-Glycine gel range from 2-40 kDa (~2、~4.5、~8、~13、~20、~30、~40 kDa), of which 30 kDa and 8 kDa are rose-red bands, others are blue bands. which is convenient for monitoring of electrophoresis and transfer efficiency. It is suitable as a protein molecular weight standard for SDS-PAGE and Western Blot. 

 Shipping and Storage Conditions

 Ship with wet ice; Store at -20℃, valid for 12 months.

Descriptions

Product Information

Product Description

Save time and effort, eliminate errors:
No need to re-gel, sample and electrophoresis; save precious samples; eliminate re-sampling errors.

Quick and efficient:
Incubate for 5-15 minutes at room temperature to strip antibody.

Mild formulation:
Does not interfere with protein re-assay after elution.

Odorless: 
Thiol-free, no offensive odors.

Ready and easy to use:
No dilution necessary, ready to use with open cap.

Storage and Shipping Conditions

Ship with wet ice, store at 2-8℃, valid for 12 months.

Components

Assay Protocol

1. TBST에 5분 간 한번 Wash 

2. TBST 부어 내고 NC 또는 PVDF가 덮일 만큼 적절량의 Stripping buffer 첨가

3. RT에서 5~15분 간 천천히 shaking

3. Antibody Stripping Buffer제거하고 TBST로 5분간 3번 Wash

4. 이후 실험 진행 (blocking – washing – 항체 반응 등)

Note

1. This product is corrosive, take caution when using it.

2. Reuse is not recommended.

3. For your safety and health, please wear safety glasses, gloves, or protective clothing.

Table 1: Reference for antibody stripping buffer selection

For Research Use Only!

Descriptions

Product Information

Product Description/Introduction

Coomassie Brilliant Blue Ultrafast Staining solution is a staining solution with Coomassie brilliant blue G250 as the dye. It can be used to stain protein gels such as SDS-PAGE or non-denaturing PAGE, and can also be used to detect residual proteins on PAGE gels after Western Blot. The staining solution is free of methanol and acetic acid, which are toxic in conventional stains; it is pollution-free, non-toxic, non-irritating odor, highly environmental protection; No fixation, shorter staining time and high sensitivity with this stain; 50 ng of protein can be detected in half an hour for a 1.0 mm thick gel; 20 ng of protein can be detected in as little as 1 hour of staining. After staining, protein bands can be observed with or without washing for 30 min.

Storage and Shipping Conditions

Ship at room temperature; Store in the dark at 2-8℃, valid for 12 months.

Product Components

Assay Protocol/Procedures

1. Remove the polyacrylamide gel after electrophoresis, put it into a clean vessel, clean it with pure water three times, 20 s each time；

2. Pour out pure water, add 20 mL of Coomassie brilliant blue ultrafast staining solution (to submerge the gel), and shake for 30 min (the gel staining time can be adjusted in real time according to the depth of the protein bands)；

3. Pour out the staining solution and wash with pure water by shaking for 30 min. The longer the decolorization time, the lighter the background will be (adjust the decolorization time according to the experimental needs). If the gel with lower background is needed, the gel can be decolorized with pure water for more than 1 h or overnight.

Note

1. The stain will settle at the bottom of the bottle after a period of time. Please mix it upside down before use.

2. The staining solution is acidic and slightly corrosive. Please take protective measures.

3. Cleaning with pure water before dyeing can greatly reduce the interference of SDS.

4. Take photos quickly after dyeing. Long immersion in pure water will lead to the loss of protein and dye.

5. For your safety and health, please wear safety glasses, gloves, or protective clothing.

For Research Use Only!

Descriptions

전기영동 기술은 생명과학 연구분야 및 분석화학, 임삭화학, 독성학, 면역학 등에서 널리 알려진 실험방법입니다.

이 방법은 저분자물질들을 분리해서 분석할 때 많이 사용하며, 단백질, 핵산, 엔자임, 바이러스 세포 등을 연구할 때도 많이 사용됩니다. 전기영동은 단백질을 분리해서 정량할 때 뿐 아니라 다양한 면학학 실험에도 사용됩니다.  

– 안정적이고 안정적인 성능을 위해 별도의 겔 준비 모드를 채택

– Bio-Rad, Servicebio사의 다양한 모델과 호환 가능

– 서비스바이오만의 독창적인  gel making system 디자인은 매우 컴팩트하고 공간활용도가 높음

– 다양한 두께의 glss plate와 샘플 comb (0.75mm , 1mm, 1.5mm) 을 사용할 수 있음

– 동시에 2개에서 4개까지 gel을 만들 수 있음

– BVT-4나 BVT-2 transfer system과 함께 사용할 수  있음

Product Features

촬영시 3가지 모드로 손쉽게 촬영이 가능하고, 다양한 파라메터를 직접 조정해서 더욱 깔끔하고 명확한 이미지 획득이 가능합니다.
UV laser protective plate가 있어서 gel cutting을 할때 편리하게 그리고 안전하게 할 수 있습니다.
 

Technical Specifications and Precautions 

Functional Description

Operating Procedures

Power On

Plug in the power cord and turn on the power switch at the back of the instrument. The industrial computer will start up.

Sample Loading

Protein Gel: Open the instrument door, take out the white sample tray, place the prepared text sample on the tray, and then place the tray flat in the groove inside the instrument dark box. Close the instrument door.

Nucleic acid gel: Open the instrument door, place the sample on the UV-transmitting glass of the UV light source module. There are markings on the UV glass surface indicating the sample placement area.

Launching the Imaging Software

Preview and Capture Pages

The preview and capture page includes status display information,  camera settings, lens settings,  mode settings, image saving and cropping, etc.

The top bar includes UV light source status indicator, keyboard open button, minimize, window restore, exit gel imaging; file path input for saving the location of the image, file name input for saving the name of the image.

The middle window displays real-time images taken by the camera.

The bottom bar includes camera/control board/mobile disk connection status display information,  image format selection for saving (TIFF/JPEG/BMP), save image button, open image path, image crop button.

The right sidebar is for camera settings, mode settings, and lens settings area, details are as follows:

Video Settings Double-clicking “Video Setting” will center the image

Contrast    Brightness  adjustment,  range  -100~ 100,  default value  0,  increasing  the value  makes  the  image brighter, decreasing the value makes the image darker

Exposure Set exposure time in milliseconds, default time 350ms, increasing the time makes the image brighter Gain Set gain value, default value 500%, range 100%~5000%, increasing the gain makes the image brighter

Auto Exposure Automatic exposure mode, the camera automatically adjusts exposure time and gain Manual Exposure Manual exposure mode, manually set exposure time and gain

Inverse Color Black and white color inversion

Lighting Turn on/off the LED lighting, used for shooting protein gel mode

UV Light  Turn on/off the UV light source, can only operate on/off when the door is closed, used for shooting nucleic acid gel mode

Gel Cutting Turn on/off the gel cutting mode, can only operate on/off when the door is open, when opening gel cutting, the UV light source will also automatically turn on, used for cutting gel, the UV protection shield must be placed before opening gel cutting

Adjustment Mode Select the zoom, focus, and aperture adjustment range of the electric lens

Coarse Adjustment   Larger adjustment steps, zoom adjustment step 200, focus adjustment step 1000, aperture adjustment step 200, when the image is completely blurry, coarse adjustment can quickly make the image relatively clear

Fine Adjustment  Moderate adjustment steps, zoom adjustment step 50, focus adjustment step 250, aperture adjustment step 50, after coarse adjustment, fine adjustment can make the image clearer

Super Fine Adjustment  Smaller adjustment steps, zoom adjustment step 5, focus adjustment step 25, aperture adjustment step 5, can make the image in the clearest state

Zoom  Increase and decrease field of view by clicking “-” and “+” buttons Focus Adjust image clarity by “-” and “+” buttons

Aperture Adjust image brightness by “-” and “+” buttons

Capturing Process

1.Select automatic exposure, default contrast is 0, default exposure is 350, and default gain is 500 .

2. For nucleic acid gel: Click on the UV light, turn on the UV light source, then the bands can be seen in the window.

For protein gel: Click on the illumination, turn on the bright field light source, then the bands can be seen in the window.

3.Adjust the “zoom” to fill the field of view with bands, or adjust to the experimenter ‘s desired field of view.

4.Adjust the “aperture,” first adjust to the maximum, then click ” — ” twice for fine adjustment, and the aperture is set to 3 .

5.Adjust the “focus ” to make the image as clear as possible; in rare cases, if the results of fine adjustment and super-fine adjustment are still  not clear enough, adjust to  reduce the  “aperture,” then focus to obtain the clearest image.

6.Enter the file path and file name, click “Save Image” to save the current image.

6. If only part of the bands is needed, click on ” image crop” to enter the image cropping page. Slide the screen from the upper left corner of the desired band to its lower right corner, and a red box will appear to outline the band. Double-click on any position inside the red box to zoom in the band to fill the entire window; Horizontal flip and vertical flip can be used to flip the band along the horizontal and vertical directions, while restore can return the band to its original state before flipping; After adjusting the band to the desired state, enter the file path and file name, then click “Save Image ” to save the current image; Click ” Exit ” to return to the preview and shooting page. If any adjustments are made to the image on the results page

Gel Cutting

1.Open the instrument door, the UV light source will automatically turn off.

2. Pull out the drawer of the UV light source, place the UV protective shield diagonally between the drawer and the instrument to shield the eyes from the UV light source.

3.Click on “Gel Cutting,” the UV light source will turn on. At this point, the bands of the nucleic acid gel can be observed, and the gel cutting action can be performed.

Product Packing List

Descriptions

SCG-W3000 Chemiluminescence Imaging System

1. Whether the signal is weak or strong, a single imaging session can deliver your WB results.

• For weak signals, you can directly extend the imaging time based on the original image for further imaging.

• For strong signals, you can adjust the timeline to retrieve any result image from the imaging duration.

2. Supports simultaneous imaging of up to 10 membranes (or even more), improving instrument efficiency—1 device equals 10!

3. Dual-camera system:

• A color camera captures markers with true-to-life colors for greater authenticity.

• A monochrome camera captures bands with high sensitivity, delivering superior performance for weak signals.

4. Marker optimization function:

• Eliminates nonspecific band signals caused by prestained markers and antibody interactions.

• Adjusts overexposed or underexposed chemiluminescent marker signals.

5. Source file storage allows flexible adjustments anytime:

• If bands appear too thick or too thin after the experiment, you can easily fine-tune the results on your computer using the source file function!

Work with the ECL Chemiluminescence Kit (G2161-200ML), it meets the exposure needs of high, medium, and low-concentration samples. Enjoy a better WB imaging process and achieve superior WB imaging results!

SWE Imaging Grayscale Analysis Software-SCG-W2000 SCG-W3000-3.3.2-win32-x64-setup.exe

Download link from Google Drive:https://drive.google.com/file/d/1IoXazML9PDHoN4HqOYm-t3C2bVFvQ_rt/view?usp=drive_link

SOFTWARE USER MANUAL:

SWE Imaging Grayscale Analysis Software-SCG-W2000 SCG-W3000.pdf

Notes:

1. Do not touch or scratch the internal lens of the dark box with hands or sharp objects.

2. After placing the sample, ensure the instrument door is properly closed to prevent external light from entering and affecting results.

3. During imaging, avoid shaking the workbench or instrument to prevent compromised image quality.

4. Observe electrical safety—do not pull the power cord or move the instrument during operation.

5. After experiments, clean the dark box thoroughly, removing all samples and residues.

Parameter Comparison

Specifications

Operation Steps
Plug the power cord of the grinder into a grounded 220V power outlet, and turn on the power switch at the back of the grinder.
After the startup interface is displayed, wait for about 5 seconds to enter the main operating interface.
Once the grinder is turned on, the cooling system starts running. Place the grinding tubes containing the samples, tighten the adapter, and cover the top of the grinder.
Set the operating frequency, time, and number of cycles. Click the “Temperature Selection” button to choose the temperature mode and set the desired temperature for the grinding chamber or the cooling platform.
Click the start button to begin the grinding process. The instrument will automatically stop once the timer reaches zero.

Equipment Maintenance
Perform the following routine maintenance after each use to ensure the reliable operation of the grinder.
Make sure to turn off the power and unplug the power cord before cleaning.
If solvents, salt water, acidic or alkaline solutions are spilled on the grinder, immediately wipe them clean with a damp cloth to avoid damaging the instrument.
Do not use high-pressure sterilization on any part of the grinder, including the adapter.
The components of the adapter should be cleaned after use. Use an appropriate cleaning agent, rinse with distilled water, and then wipe dry with a paper towel.
After using an appropriate cleaning agent, wipe the grinder clean with a soft cloth.
The following disinfectants and cleaning agents are recommended for cleaning the grinder and adapter:
a) Ordinary cleaning agent
b) Neutral detergent
c) 70% alcohol content 

 Equipment List

DNA Extraction

(This condition is for reference only, and can be adjusted at any time during the experiment according to the grinding effect.)

 RNA Extraction

(This condition is for reference only, and can be adjusted at any time during the experiment according to the grinding effect.)

For RNA extraction, the grinding time is extended compared with that of protein extraction, and RNA extraction solution is added to the grinding, the specific grinding time depends on the amount and type of tissue to be ground. Samples difficult to break can be repeated grinding 2-3 times, the specific situation can be adjusted appropriately according to the experiment.
Strong and Tough Tissue Grinding
For samples that are difficult to grind (e.g. cartilage, hair, etc.), we recommend using a 2mL grinding tube. Cut the sample into 1-2mm pieces, add the appropriate number of grinding beads, cover the tube tightly and freeze in liquid nitrogen for 2-3min without extracting solution, dry grind for 60 seconds at 70Hz, then add extracting solution to the tube and repeat the grinding once. If the grinding result is not satisfactory, the tube can be frozen again and the above experimental steps can be repeated.
Bone Tissue Grinding
For bone tissue, use the special steel grinding jar and adapter. The bone tissue is first frozen in liquid nitrogen for 5-10min, then put into the grinding jar, add the steel beads, and grind for 180 seconds at 70Hz (set cycle grinding). After grinding, take out the finished tissue, put it into a 2mL grinding tube, add the extraction solution, two 3mm grinding beads and homogenize for 60 seconds.      

Descriptions

이 키트는 매우 간단하고 빠르고 원한는 %의 SDS-PAGE color gel을 만들 수 있는 제품입니다. 동시에 resolving과 stacking gel을 만들 수 있으며, 만든 gel은 1주일간 보관 가능합니다. 해당 gel은 Tris-glycin runining buffer를 사용해야 합니다.

Stacking gel reagent 안에는 red 또는 green dye가 들어 있으며, 이는 샘플 로딩 시 각각의 gel을 명확하게 구분지어 손쉽게 로딩 가능합니다. 해당 dye는 resolving gel로 이동하지 않으므로, 전기영동 이후 다음 실험에 전혀 영향을 끼치지 않습니다.

추가로 독성이 강한 TEMED 대신 더욱 안전한 대체제를 사용하여 연구자를 더욱 안전하게 보호합니다.

Storage

Store at 4℃, the shelf life is 1 year.

제품

 G2041-50T      Red, 6%

 G2042-50T      Red, 8%

 G2043-50T      Red, 10%

 G2044-50T      Red, 12%

 G2045-50T      Red, 15%

 G2060-50T      Green, 6%

 G2061-50T      Green, 8%

 G2062-50T      Green, 10%

 G2063-50T      Green, 12%

 G2064-50T      Green, 15%

아래는 6% 제품을 바탕으로 넣은 예입니다.

Component

Assay Protocol / Procedures

1. Choose appropriate Concentration of acrylamide solution according to molecular weight of target protein;

2. Prepare 10% APS solution:100 mg of APS powder is dissolved in 1.0 mL of ultrapure water to obtain a 10% APS solution;

Note: 10% APS solution can be stored at 4°C for 1-2 weeks, and APS powder can be stored at -20°C.

3. Preparation of 6% resolving gel acrylamide solution:in a suitable container,add equal volumes of 6% Resolver A and 6% Resolver B;

4. Add the required volume of freshly made 10% APS to the combined resolver solution and mix well. Use an appropriate pipet to steadily dispense the solution into the cassette. Do not let bubbles form or solution mix with air. Fill the cassette to 0.5-1 cm below the bottom of the teeth on the comb. Immediately and slowly add pure water or ethanol to the cassette. Allow the resolver to polymerize for 15-30 min, then pour water or ethanol out;

 Alternative:pour the stacking solution instead of water or alcohol as directed in the next steps.

5. Prepare stacking gel acrylamide solution by combining equal volumes of stacker A and B solution,then add required volume of freshly made 10% APS to the combined stacker solution and mix well.Pipet solution down the middle of the cassette, filling to the top of the short plates. Apply slowly and steadily to prevent mixing with the resolving solution. Align and insert the comb in the cassette carefully to prevent air from being trapped under the comb teeth;

6. After adding APS to the stacking solution mixture, casting should be started immediately and combs inserted within 8 min;

7. Allow the gel to polymerize for 15–30 min before electrophoresis;

8. The cast gels can be covered with a paper towel wetted with deionized water and stored in a zipper sealed plastic bag at 4℃ for up to one week.

Note

1. There is monomer acrylamide in the premix, which is harmful to human body. Please pay attention to protective measures during operation;

2. Pour the gel as soon as possible after adding APS, do not leave it for a long time;

3. There is a significant positive correlation between the solidification and the temperature of the gel. Under the same conditions, the higher the temperature, the faster the solidification rate.

4. We also provide other colors (green, yellow, blue) of stacking solutions to distinguish different samples and different gel electrophoresis;

5. Tris-Glycine buffer system is suitable (recommend our product: G2018-1 L).

For Research Use Only!