Chemiluminescence Imaging System W3000/W5000

Descriptions

I. Introduction

SCG-W3000은 900만 화소의 고감각 냉각카메라를 탑제한 Chemiluminescence Imaging System입니다. 샘플을 빠르고, 정확하게, 여러장의 멤브레인을 동시에 촬영 가능한 장비입니다. 단백질 검출 관련된 다양한 분야에서 활용이 되며, W5000 모델의 경우 UV module까지 장착 되어 DNA gel 촬영까지도 가능합니다.

II. Features

본 제품은 다른 제품과 다르게 실시간 이미지 관찰이 가능하며, 촬영이 마친 이후에도 추가 촬영을 통해 기존 것과 accumulation되어 재촬영 없이도 간단하게 더 좋은 결과를 얻을 수 있습니다. 또한 중간에 별도의 시간 설정 없이도 time imaging기능을 통해 어느 시점이든 원하는 시점의 이미지를 얻을 수 있습니다. 
물론 다른 장비와 같이 스스로 최적의 노출값을 자동 인식하여 자동촬영 해주는 기능도 보유하고 있습니다.

Real-time Imaging:
Present the changes in sample signals during the exposure process in real-time, capturing every detail of the shot. Overexposed areas will be indicated for samples that are overexposed.

Time Imaging: 
After the exposure is completed, every frame image within the exposure time can be generated. Through precise retrospective adjustments, users can select any frame image within that exposure time as the final output.

Time Accumulation: 
For samples with insufficient exposure, users can choose to continue the exposure after the exposure ends, allowing the sample to obtain additional exposure effects based on the exposure time already accumulated.

Automatic Exposure: 
Intelligent exposure technology can quickly determine the optimal exposure time. With the functions of time imaging and time accumulation, users can obtain the best image results with just one operation.

III. Technical Specifications and Precautions 

Notes:

1. It is prohibited to touch or scratch the internal lenses of the dark box with hands or sharp objects.

2. After placing the experimental samples, make sure to close the instrument’s flip door to prevent external light from entering the dark box and affecting the experimental results.

3. During imaging experiments, shaking the experimental table or instrument is prohibited to avoid impacting the image quality.

4. Pay attention to electrical safety. Pulling or moving the power cord during the experiment is prohibited.

5. After the experiment is completed, clean the samples and any residues inside the dark box thoroughly.

IV. Functional Description

① Internal camera lens assembly, which is the core component of the imaging system.

② 10.4-inch industrial computer with user software operating interface.

③ High-quality light-shielded imaging dark box.

④ Sample tray, removable, used for placing samples to be photographed.

⑤ LED white light source for illumination.

⑥ Power socket and switch.

⑦ Cooling device.

⑧ External USB 3.0 interface.

V. Operating Procedures

1. Power On

Plug in the power cord and turn on the power switch at the back of the instrument. The industrial computer will start up.

2. Sample Loading
Open the instrument door, take out the sample tray, place the prepared text sample on the tray, and then place the tray flat in the groove inside the instrument dark box. Close the instrument door.

3. Launching the Chemiluminescence Imaging Software

After the industrial computer starts up, the chemiluminescence application software will be automatically loaded. Once the software is successfully launched, it will navigate to the main page.

The top-left section displays the company logo.

The bottom-left section is the status bar, showing the current camera and control board connection status, as well as detection of the inserted removable disk.

The top-right section displays the software version number.

The bottom-right section includes buttons for switching between simplified/traditional Chinese, switching to English, exporting the page, and closing the program.

Clicking on the central icon will enter the preview and capture page.

4. Software Self-Check
If the software starts abnormally, or if the camera or control board is not connected, the text in the software system will turn gray, and the central icon will also become gray. The self-check completion prompt will turn red, and it will not be possible to click into the preview and exposure page. At this time, you need to check whether the camera and PCB board drivers are correctly installed.

5. Preview and Capture Pages

In the preview page, you need to enter the location where the experimental results will be stored. The file name is an optional input to facilitate file searching for the user.

The user can choose between manual exposure and automatic exposure as the current experimental mode. For manual exposure, the user needs to input the exposure time for the exposure. For automatic exposure, the algorithm calculates the optimal exposure time.

On the right side of the preview page, there is a time input field in microseconds (us), which represents the exposure time for the bright field image. Clicking the preview switch will start the preview, and the preview time can be adjusted as needed.

Shooting mode:High quality, standard, high sensitivity. You can choose a mode to shoot according to your needs, the high-quality mode takes the best image quality, the standard mode takes into account the image quality and shooting time, and the high-sensitive mode takes the shortest shooting time.

Clicking the capture button will initiate the capture and exposure process, while clicking the back button will return to the main page.

Preview and Capture Pages

6. Capturing Process

(1) Select automatic exposure and set preview time.

Automatic exposure: Intelligent exposure technology can quickly determine the optimal exposure time.

(2) Real-time imaging.

Click the capture button to start the exposure. The left side displays the stripe, while the right side shows the countdown of the exposure time. As the countdown progresses, the stripe on the left side will show real-time imaging.

Real-time imaging: Real-time display of changes in sample signal during the exposure process, enabling users to grasp every detail of the capture process. This breakthrough function not only improves capture efficiency but also greatly enhances user interaction experience.

During the real-time imaging process, any overexposed areas in the stripe will be displayed in red. If the stripe is judged to meet requirements, the user can click the stop button in the lower right corner to end the exposure early.

Real-time imaging

(3) Temporal imaging, temporal accumulation.

After the exposure is completed, it automatically enters the results page where adjustments can be made to the captured results.

Time imaging:Through precise retrospective adjustments, users can select any frame within the exposure time as the final output result.

Time accumulation:Even after the exposure is completed, users can choose to continue exposure to obtain additional exposure effects based on the already exposed time. When clicking on “continue exposure,” the user will be prompted with the minimum exposure time. The set time needs to be greater than this minimum time. If the set time is shorter than this minimum time, the actual exposure time will be the suggested minimum exposure time.

Figure 4.5 Temporal imaging

Temporal accumulation

(4) Image adjustments, result saving. After obtaining satisfactory images through automatic exposure, continued exposure, and temporal imaging, adjustments can be made to the bright field image, dark field image, and composite image on the results page.

①　Contrast:Adjust the brightness and darkness of the bright field image.

②　Exposure:Adjust the lightness and darkness of the dark field image. Clicking on “auto-adapt” can recommend the most suitable value.

③　Rotation: Rotate the image.

④　Invert:Apply an inverted color effect to the image, turning black into white and white into black.

⑤　Crop: Clicking on crop will generate a region on the image. This region can be resized, and after selecting the appropriate size, clicking on crop again will crop out the desired area.

⑥　Vertical flip:Flips the image vertically.

⑦　Horizontal flip:Flips the image horizontally.

⑧　Restore:Restores the image to its original state.

⑨　Import: Select the file automatically saved by the program to import it into the program for further operations.

⑩　Image saving: Save the image and also save the bright field image, dark field image, and composite image.

⑪　Result saving: Save the adjusted image as an encrypted file. 

If any adjustments are made to the image on the results page

 Bright field image

Dark field image

Composite Image

Result saving

7. Export Page

                                                 Export page

① Storage location: Select the location of the image files automatically saved by the system, and the contents of the files will be displayed in a list format in the system.
② Open file: Open the selected directory.
③ Export: Select the export content and format to export the images.
④ Delete: Select the image file to be deleted, and it will be deleted.
⑤ Open: Select the corresponding file in the list to open and jump to the result page. You can operate on the image on the result page, or double-click the corresponding file to open it.
⑥ Return: Click “Return” to return to the main page.

Specifications

Operation Steps
Plug the power cord of the grinder into a grounded 220V power outlet, and turn on the power switch at the back of the grinder.
After the startup interface is displayed, wait for about 5 seconds to enter the main operating interface.
Once the grinder is turned on, the cooling system starts running. Place the grinding tubes containing the samples, tighten the adapter, and cover the top of the grinder.
Set the operating frequency, time, and number of cycles. Click the “Temperature Selection” button to choose the temperature mode and set the desired temperature for the grinding chamber or the cooling platform.
Click the start button to begin the grinding process. The instrument will automatically stop once the timer reaches zero.

Equipment Maintenance
Perform the following routine maintenance after each use to ensure the reliable operation of the grinder.
Make sure to turn off the power and unplug the power cord before cleaning.
If solvents, salt water, acidic or alkaline solutions are spilled on the grinder, immediately wipe them clean with a damp cloth to avoid damaging the instrument.
Do not use high-pressure sterilization on any part of the grinder, including the adapter.
The components of the adapter should be cleaned after use. Use an appropriate cleaning agent, rinse with distilled water, and then wipe dry with a paper towel.
After using an appropriate cleaning agent, wipe the grinder clean with a soft cloth.
The following disinfectants and cleaning agents are recommended for cleaning the grinder and adapter:
a) Ordinary cleaning agent
b) Neutral detergent
c) 70% alcohol content 

 Equipment List

DNA Extraction

(This condition is for reference only, and can be adjusted at any time during the experiment according to the grinding effect.)

 RNA Extraction

(This condition is for reference only, and can be adjusted at any time during the experiment according to the grinding effect.)

For RNA extraction, the grinding time is extended compared with that of protein extraction, and RNA extraction solution is added to the grinding, the specific grinding time depends on the amount and type of tissue to be ground. Samples difficult to break can be repeated grinding 2-3 times, the specific situation can be adjusted appropriately according to the experiment.
Strong and Tough Tissue Grinding
For samples that are difficult to grind (e.g. cartilage, hair, etc.), we recommend using a 2mL grinding tube. Cut the sample into 1-2mm pieces, add the appropriate number of grinding beads, cover the tube tightly and freeze in liquid nitrogen for 2-3min without extracting solution, dry grind for 60 seconds at 70Hz, then add extracting solution to the tube and repeat the grinding once. If the grinding result is not satisfactory, the tube can be frozen again and the above experimental steps can be repeated.
Bone Tissue Grinding
For bone tissue, use the special steel grinding jar and adapter. The bone tissue is first frozen in liquid nitrogen for 5-10min, then put into the grinding jar, add the steel beads, and grind for 180 seconds at 70Hz (set cycle grinding). After grinding, take out the finished tissue, put it into a 2mL grinding tube, add the extraction solution, two 3mm grinding beads and homogenize for 60 seconds.