Fast-Cast Colorful Acrylamide Kit

Descriptions

이 키트는 매우 간단하고 빠르고 원한는 %의 SDS-PAGE color gel을 만들 수 있는 제품입니다. 동시에 resolving과 stacking gel을 만들 수 있으며, 만든 gel은 1주일간 보관 가능합니다. 해당 gel은 Tris-glycin runining buffer를 사용해야 합니다.

Stacking gel reagent 안에는 red 또는 green dye가 들어 있으며, 이는 샘플 로딩 시 각각의 gel을 명확하게 구분지어 손쉽게 로딩 가능합니다. 해당 dye는 resolving gel로 이동하지 않으므로, 전기영동 이후 다음 실험에 전혀 영향을 끼치지 않습니다.

추가로 독성이 강한 TEMED 대신 더욱 안전한 대체제를 사용하여 연구자를 더욱 안전하게 보호합니다.

Storage

Store at 4℃, the shelf life is 1 year.

제품

 G2041-50T      Red, 6%

 G2042-50T      Red, 8%

 G2043-50T      Red, 10%

 G2044-50T      Red, 12%

 G2045-50T      Red, 15%

 G2060-50T      Green, 6%

 G2061-50T      Green, 8%

 G2062-50T      Green, 10%

 G2063-50T      Green, 12%

 G2064-50T      Green, 15%

아래는 6% 제품을 바탕으로 넣은 예입니다.

Component

Assay Protocol / Procedures

1. Choose appropriate Concentration of acrylamide solution according to molecular weight of target protein;

2. Prepare 10% APS solution:100 mg of APS powder is dissolved in 1.0 mL of ultrapure water to obtain a 10% APS solution;

Note: 10% APS solution can be stored at 4°C for 1-2 weeks, and APS powder can be stored at -20°C.

3. Preparation of 6% resolving gel acrylamide solution:in a suitable container,add equal volumes of 6% Resolver A and 6% Resolver B;

4. Add the required volume of freshly made 10% APS to the combined resolver solution and mix well. Use an appropriate pipet to steadily dispense the solution into the cassette. Do not let bubbles form or solution mix with air. Fill the cassette to 0.5-1 cm below the bottom of the teeth on the comb. Immediately and slowly add pure water or ethanol to the cassette. Allow the resolver to polymerize for 15-30 min, then pour water or ethanol out;

 Alternative:pour the stacking solution instead of water or alcohol as directed in the next steps.

5. Prepare stacking gel acrylamide solution by combining equal volumes of stacker A and B solution,then add required volume of freshly made 10% APS to the combined stacker solution and mix well.Pipet solution down the middle of the cassette, filling to the top of the short plates. Apply slowly and steadily to prevent mixing with the resolving solution. Align and insert the comb in the cassette carefully to prevent air from being trapped under the comb teeth;

6. After adding APS to the stacking solution mixture, casting should be started immediately and combs inserted within 8 min;

7. Allow the gel to polymerize for 15–30 min before electrophoresis;

8. The cast gels can be covered with a paper towel wetted with deionized water and stored in a zipper sealed plastic bag at 4℃ for up to one week.

Note

1. There is monomer acrylamide in the premix, which is harmful to human body. Please pay attention to protective measures during operation;

2. Pour the gel as soon as possible after adding APS, do not leave it for a long time;

3. There is a significant positive correlation between the solidification and the temperature of the gel. Under the same conditions, the higher the temperature, the faster the solidification rate.

4. We also provide other colors (green, yellow, blue) of stacking solutions to distinguish different samples and different gel electrophoresis;

5. Tris-Glycine buffer system is suitable (recommend our product: G2018-1 L).

For Research Use Only!