GB11001 Anti-beta Actin Rabbit pAb
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility .
Descriptions
|
Description |
beta Actin rabbit polyclonal antibody |
|
Protein full name |
Actin, cytoplasmic 1 |
|
Synonyms |
ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse β-Actin |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
Cytoplasm |
|
Predicted MW. |
42 kDa |
|
Observed MW. |
45 kDa |
|
Uniprot ID |
P60710 |
Applications
|
Applications |
Species |
Dilution |
Positive Sample |
|
WB |
Human, Mouse, Rat |
1: 1000-1: 2000 |
lung, kidney, brain |
|
IHC/IF |
Human, Mouse, Rat |
1: 500-1: 1000 |
colon cancer, liver |
|
ICC/IF |
Human, Mouse, Rat |
1: 300-1: 1000 |
NIH/3T3, A549, etc. |
|
|
Western blot analysis of beta-Actin (GB11001) at dilution of 1: 1000 |
|
|
Immunofluorescence of paraffin embedded human colon cancer using beta-Actin (GB11001) at dilution of 1: 1000 |
Storage
|
Storage |
Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
|
Storage Buffer |
PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.