Hypersensitive ECL Chemiluminescence Kit
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Descriptions

Product Information

Product Description/Introduction

ECL chemiluminescence Kit is a highly sensitive chemiluminescence kit based on luminol ECL chemiluminescence. This product can react with horseradish peroxidase (HRP) conjugated on secondary antibody to produce fluorescence and detect samples by using X-ray lithography or other appropriate fluorescence imaging equipment (fluorescence or chemiluminescence imaging system, etc.). Low concentration samples can still be detected, saving samples. Signal can still be detected after incubation with low concentrations of antibodies (primary, secondary), saving precious antibodies. Signal values are enhanced at least 10-fold compared to the Sensitive ECL Chemiluminescence Kit (G2074).

Storage and Shipping Conditions

Ship with wet ice; Store in the dark at 2-8℃, valid for 12 months.

Product Components

Assay Protocol/Procedures

1. hypersensitive ECL working solution 준비: the hypersensitive ECL solution A와 hypersensitive ECL solution B  같은 볼륨으로 섞음. 해당 솔루션은 실온에서 바로 사용 가능합니다.

2. Western 실험에서, PVDF membrane (또는 NC membrane)는 2nd Ab 반응 시키고, 몇 번의 washing과정을 거친 후에,  위에서 섞은 ECL working solution이 membrane을 덮을 정도로 넣은 후 1~2분간 반응 시킵니다.

3.  ECL working liquid를 filter paper나 absorbent paper로 흡수 시켜 제거하거나, membrane을 꺼내서 촬영을 위한 비닐 판 또는 보드판에 놓아 줍니다.

4.  pressed film에 사용한다면 develop과 fixed과정을 거칩니다.(G2019, G2023, G2024추천합니다.). 촬영을 합니다.

Note

1. Be sure to change the pipette tips during pipetting of Reagent A and B to avoid cross-contamination, which may result in the failure of active components.

2. Please wear gloves and use clean equipment such as tweezers to avoid contamination by exogenous proteins and metal ions during contact with the membrane.

3. Sodium azide inhibits HRP activity and all related reagents should be avoided.

4. Please use up the ECL working solution within one day after it has been configured, do not leave it until the next day, so as not to affect the accuracy of the experimental results.

5. After the use of each solution, please keep the bottle tightly capped and keep it away from light to prevent failure; especially solution B, which contains oxidizing agents and is easily reduced and become ineffective. In addition, liquid A should not be stored close to the inner wall of the refrigerator to prevent the precipitation of crystals due to the local temperature is too low. If crystals are precipitated, dissolve and mix in 37℃ warm water bath before use.

6. ECL chemiluminescence reagent kit selection refer to Table 1, primary antibody and secondary antibody recommended dilution ratio test data are from self-research antibody.

7. Refer to Table 2 for common problems and solutions for ECL chemiluminescent assays.

8. ECL chemiluminescence kit A/B solution are harmful to human body, must be careful when handling, pay attention to effective protection, avoid direct contact with the human body or inhalation of the respiratory tract.

9. For your safety and health, please wear safety glasses, gloves, or protective clothing.

Table 1: ECL Chemiluminescent Kit Selection Reference Table

Table2:Common problems and Solutions of ECL Chemiluminescence Detection