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Tissue Homogenizer Low Temperature (-50℃, Freezing Table)

SWE-FP는 -50℃까지 챔버가 냉동되는 저온 비드 호모게나이저입니다. 그 뿐 아니라 -20℃까지 내려가는 저온 테이블이 장착되어 있습니다. 수직 운동을 통한 고속 왕복운동 방식의 기술을 적용했으며, 얼려진 샘플이 비드와의 충돌로 인해 파괴되어 완벽하게 조직을 분쇄합니다.

최대 192개의 샘플을 동시에 분쇄시킬 수 있으며, 대용량(50ml)이 샘플까지도 분쇄 가능한 대용량, 많은 처리량을 갖춘 장비입니다.

 

Parameters Comparsion

Cat. No. KZ-III KZ-III-F/FP KZ-5F-3D SWE-FP
Operating mode The vertical shaking system  the vertical shaking system Move in a “∞” shaped three-dimensional motion in space. The vertical shaking system 
Temperature Room temperature -10℃/-40℃ -40℃ -50℃
Temperature control precision N/A ±1℃ ±1℃ ±1℃
Standard spec. 24*2 mL 24*2 mL 24*2 mL 24*2 mL
Maximum sample volume 60 pcs 60 pcs 24 pcs 192 pcs
Adapter specification 24×0.5mL. 24x2mL. 32x2mL. 48x2mL. 60x2mL. 12x5mL. 10x10mL. 6x5mL (steel can). 4x30mL (steel can). 2x50mL (steel can) and 8*15mL (steel can) 24*2mL.
12*5mL (Optional )
24×0.5mL. 24x2mL. 48x2mL. 32x2mL. 60x2mL. 96x2mL. 12x5mL. 10x10mL. 4x30mL (steel can). 6x5mL (steel can). 8x15mL (steel can). 
2x50mL (steel can). 96x2mL (2pcs 96-deep well plate)
Freezing table N/A N/A N/A YES
Adapter fixing method Nut Nut Knob cover with safety locking device Nut
Working time 0-99min99s 0-99min99s 0-99min99s 0-99min99s
Dimensions 365*265*396mm 370*365*500mm 570*393*382mm
Screen size 5 inches 5 inches 5 inches 5 inches
Emergency stop button YES YES YES YES
Electromagnetic safety lock Screen click control with automatic power-off unlocking. the cover cannot be opened during operation until the grinding program ends, fully protected.
Maximum feeding size No requirements, adjustable according to the adapter.
Weight 30.7kg 45kg 52kg 54kg
Power parameters 200-240VAC, 50-60 Hz, 200 W 200-240VAC, 50-60 Hz,
400 W
200-240V AC,50-60 Hz,
400 W
200-240VAC,50-60 Hz,500 W
Cooling method none Compressor cooling Compressor cooling Compressor cooling
Transport screws Yes (2 at the bottom) Yes (2 at the bottom) N/A Yes (2 at the bottom)
Advantages

Small size,

cost-effective

Compatible with various adapter specifications.
Freezing function, lowest temperature can reach -40℃.
Higher grinding efficiency, reducing tissue degradation. Freezing function, lowest temperature can reach -40℃. Lower cooling temperature can reach -50℃. low temperature freezing table,
Protects samples and meets low-temperature needs for Western Blot experiments, electrophoresis, and transfer membranes.

Descriptions

KZ-III는 Room temperature상에서 조직을 분쇄하는 방식의 경제적인 모델입니다.

  • Product Features

    1. Slider drive, no wear shaft, noise stability and durability;
    2. Equiped with low temperature freezing table. It can meet the needs of sample storage, electrophoresis, membrane transfer and other low-temperature experiments
    3. Imported compressor,lowest cooling temperature of -50℃,low temperature grinding throughout the process,reducing degradation of proteins and RNA.
    4. Up to 192 samples can be processed simultaneously in 1 minute (24 samples can be processed with standard),efficiently and effectively completing sample grinding with high throughput.
    5. Multiple adapters to choose.

    Technical Parameters


    1. Dimensions:Length 650mm,Width 420mm,Height 430mm
    2. Weight:54 kg
    3. Screen size:5 inches
    4. Temperature:-50℃
    5. Temperature control precision:±1℃
    6. Working time:Runtime0-9999s,pausetime0-999s,andruntimes1-99times

Specifications

Operation Steps
Plug the power cord of the grinder into a grounded 220V power outlet, and turn on the power switch at the back of the grinder.
After the startup interface is displayed, wait for about 5 seconds to enter the main operating interface.
Once the grinder is turned on, the cooling system starts running. Place the grinding tubes containing the samples, tighten the adapter, and cover the top of the grinder.
Set the operating frequency, time, and number of cycles. Click the “Temperature Selection” button to choose the temperature mode and set the desired temperature for the grinding chamber or the cooling platform.
Click the start button to begin the grinding process. The instrument will automatically stop once the timer reaches zero.

Equipment Maintenance
Perform the following routine maintenance after each use to ensure the reliable operation of the grinder.
Make sure to turn off the power and unplug the power cord before cleaning.
If solvents, salt water, acidic or alkaline solutions are spilled on the grinder, immediately wipe them clean with a damp cloth to avoid damaging the instrument.
Do not use high-pressure sterilization on any part of the grinder, including the adapter.
The components of the adapter should be cleaned after use. Use an appropriate cleaning agent, rinse with distilled water, and then wipe dry with a paper towel.
After using an appropriate cleaning agent, wipe the grinder clean with a soft cloth.
The following disinfectants and cleaning agents are recommended for cleaning the grinder and adapter:
a) Ordinary cleaning agent
b) Neutral detergent
c) 70% alcohol content 

 Equipment List

No.

Product Name

Model/Specification

Quantity

Remarks

1

Tissue Homogenizer

SWE-FP

1

2

Standard Hollow Adapter

2mL*24

1

Can process 24 samples at once

3

Beads Pen

SYM-04Z

1

Suitable for 2mL grinding tubes, zirconia beads 4mm

4

Beads Pen

SYM-04B

1

Suitable for 2mL grinding tubes, steel beads 4mm

5

Beads Pen

SYM-03B

1

Suitable for 2mL grinding tubes, steel beads 3mm

6

2mL Centrifuge Tube

1

Tube wall is thickened, material strength is higher, and there is no RNA enzyme.

DNA Extraction

Product Name

Grinding Beads

Frequency

Grinding Time

Pause Time

Cycle times

Recommended Extraction Solution

Plant Stems, Leaves

Stainless Steel Beads 3mm*3, 4mm*1

60Hz

60s

15s

3

Protein Extraction Solution

Heart, Liver, Spleen, Lungs, Kidneys, Brain of Mice and Rats

Stainless Steel Beads 3mm*2, 4mm*1

60Hz

30s

15s

2

Protein Extraction Solution

Stomach, Intestines, Muscles, Fat, Pancreas of Mice and Rats

Stainless Steel Beads 3mm*2, 4mm*1

60Hz

45s

15s

2

Protein Extraction Solution

Skin, Ears, Tail, Blood Vessels of Mice and Rats

Stainless Steel Beads 3mm*3, 4mm*2

70Hz

60s

20s

3

Protein Extraction Solution

Cartilage, Hair, Hard Bones of Mice and Rats

Stainless Steel Beads 3mm*4, 4mm*2

70Hz

90s

20s

3

Protein Extraction Solution

(This condition is for reference only, and can be adjusted at any time during the experiment according to the grinding effect.)

 RNA Extraction

Product Name

Grinding Beads

Frequency

Grinding Time

Pause Time

Cycle times

Recommended Extraction Solution

Plant Stems, Leaves

Zirconia Beads 3mm*3, 4mm*1

60Hz

60s

15s

3

RNA Extraction Solution

Heart, Liver, Spleen, Lungs, Kidneys, Brain of Mice and Rats

Zirconia Beads Beads 3mm*2, 4mm*1

60Hz

30s

15s

2

RNA Extraction Solution

Stomach, Intestines, Muscles, Fat, Pancreas of Mice and Rats

Zirconia Beads Beads 3mm*3, 4mm*2

60Hz

45s

15s

2

RNA Extraction Solution

Skin, Ears, Tail, Blood Vessels of Mice and Rats

Zirconia Beads Beads 3mm*4, 4mm*2

70Hz

60s

20s

3

RNA Extraction Solution

Cartilage, Hair, Hard Bones of Mice and Rats

Zirconia Beads Beads 3mm*3, 4mm*4

70Hz

90s

20s

3

RNA Extraction Solution

(This condition is for reference only, and can be adjusted at any time during the experiment according to the grinding effect.)

For RNA extraction, the grinding time is extended compared with that of protein extraction, and RNA extraction solution is added to the grinding, the specific grinding time depends on the amount and type of tissue to be ground. Samples difficult to break can be repeated grinding 2-3 times, the specific situation can be adjusted appropriately according to the experiment.
Strong and Tough Tissue Grinding
For samples that are difficult to grind (e.g. cartilage, hair, etc.), we recommend using a 2mL grinding tube. Cut the sample into 1-2mm pieces, add the appropriate number of grinding beads, cover the tube tightly and freeze in liquid nitrogen for 2-3min without extracting solution, dry grind for 60 seconds at 70Hz, then add extracting solution to the tube and repeat the grinding once. If the grinding result is not satisfactory, the tube can be frozen again and the above experimental steps can be repeated.
Bone Tissue Grinding
For bone tissue, use the special steel grinding jar and adapter. The bone tissue is first frozen in liquid nitrogen for 5-10min, then put into the grinding jar, add the steel beads, and grind for 180 seconds at 70Hz (set cycle grinding). After grinding, take out the finished tissue, put it into a 2mL grinding tube, add the extraction solution, two 3mm grinding beads and homogenize for 60 seconds.